Journal club


Jan 18 (Iwabuchi) Heijink et al., BRCA2 deficiency instigates cGAS-mediated inflammatory signaling and confers sensitivity to tumor necrosis factor-alpha-mediated cytotoxicity. Nat. commun. 2019



Nov 2 (Sakasai) Coquel et al., SAMHD1 acts at stalled replication forks to prevent interferon induction. Nature 2018, 557, 57-61


Oct 19 (Matsui) Ghezraoui et al., 53BP1 cooperation with REV7-shieldin complex underpins DNA structure-specific NHEJ. Nature 2018, 560, 122-127


Oct 5 (Hashimoto) Dev et al., Shieldin complex promotes DNA end-joining and counters homologous recombination in BRCA1-null cells. Nat. Cell Biol. 2018, 20, 954-965


Jul 27 (Sunatani) Stewart et al., Transbilayer phospholipid movement facilitates annexin translocation across membranes. J Cell Sci. 2018, doi: 10.1242/jcs.217034


Jul 13 (Sakasai) Murai et al., SLFN11 blocks stressed replication forks independently of ATR. Mol. Cell 2018, 69, 371-384


Jun 22 (Matsui) Tomita et al., FAM35A associates with REV7 and modulates DNA damage responses of normal and BRCA1-defective cells. EMBO J. 2018, e99543


Jun 8 (Hashimoto) Nowsheen et al., L3MBTL2 orchestrates ubiquitin signalling by dictating the squential recruitment of RNF8 and RNF168 after DNA damage. Nat Cell Biol (2018) 20, 455-464


May 18 (Iwabuchi) Gupta et al., DNA repair network analysis reveals Shieldin as a key regulator of NHEJ and PARP inhibitor sensitivity. Cell 2018, 173, 972-988


Apr 27 (Sunatani) Cheng et al., ATM and ATR play complementary roles in the behavior of excitatory and inhibitory vesicle population. PNAS 2018, 115, E292-E301


Apr 13 (Sakasai) Lu et al., Drosha drives the formation of DNA:RNA hybrids around DNA break sites to facilitate DNA repair. Nat. commun. 2018 DOI: 10.1038/s41467-018-02893-x


May 30 (Matsui) Guo et al., Acetylation of 53BP1 dictates the DNA double strand break repair pathway. Nucleic Acids Res. 2018, 46, 689-703


May 13 (Hashimoto) Baranes-Bachar et al., The ubiquitin E3/E4 ligase UBE4A adjusts protein ubiquitination and accumulation at sites of DNA damage, facilitating double-strand break repair. Mol.  Cell 2018, 69, 866-878


Feb 23 (Iwabuchi) Lazarou et al., The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy. Nature 2015, 524, 309-314


Feb 2 (Sunatani) Barazzuol et al., A coordinated DNA damage response promotes adult quiescent neural stem cell activation. PLOS Biology 2017, DOI: 10.1371/journal.pbio.2001264


Dec 22 (Sakasai) Xu et al., 53BP1 and BRCA1 control pathway choice for stalled replication restart. eLife 2017; 6:e30523. DOI:


Dec 8 (Matsui) Michelini et al., Damage-induced lncRNA control the DNA damage response through interaction with DDRNAs at individual double-strand breaks. Nat. Cell Biol. 2017, 19, 1400-1411


Nov 17 (Hashimoto) Lee et al., ASF1a promotes non-homologous end joining repair by facilitating phosphorylation of MDC1 by ATM at double-strand breaks. Mol. Cell 2017, 68, 61-75


Sep 8 (Iwabuchi) Pellegrino et al., Replication-coupled dilution of H4K20me2 guides 53BP1 to pre-replicative chromatin. Cell Reports 2017, 19, 1819-1831 


Aug 25 (Sunatani) Koo et al., Nuclear TRADD prevents DNA damage-mediated death by facilitating non-homologous end-joining repair. Sci. Rep. 2017 DOI: 10.1038/s41598-017-03211-z


Jul 6 (Sakasai) Day et al., PARP3 is a promoter of chromosomal rearrangements and limits G4 DNA. Nat commun. 2017 DOI: 10.1038/ncomms15110


Jun 13 (Matsui) Ha et al., The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites. Nat commun. 2017 DOI: 10.1038/ncomms15751 


Jun 2 (Hashimoto) Biehs et al., DNA double-strand break resection occurs during non-homologous end joining in G1 but is distinct from resection during homologous recombination. Mol Cell 2017, 65 671-684


May 12 (Iwabuchi) Kucharski et al., Reciprocal regulation between 53BP1 and the anaphase-promoting complex/cyclosome is required for genomic stability during mitotic stress. Cell Reports 2017, 18, 1982-1995


Apr 13 (Sunatani) Youn et al., 53BP1 contributes to regulation of autophagic clearance of mitochondria. Sci Rep. 2017, DOI: 10.1038/srep45290


Mar 31 (Sakasai) Nguyen et al., Function of replication protein A as a sensor of R loops and a regulator of RNaseH1. Mol Cell 2017, 65, 832-847


Mar 17 (Matsui) Drané et al. TIRR regulates 53BP1 by masking its histone methyl-lysine biding function. Nature 2017 543. 211-216 


Feb 28 (Hashimoto) Zhou et al. Regulation of the DNA damage responses by DNA-PKcs inhibitory phosphorylation of ATM. Mol. Cell 2017, 65, 91-104 


Nov 25 (Iwabuchi) Kotsantis et al., Increased global transcription activity as a mechanism of replication stress in cancer. Nat. commun. 2016, DOI: 10.1038/ncomms13087


Nov 11 (Sakasai) Li et al., DEAD Box 1 facilitates removal of RNA and homologous recombination at DNA double-strand breaks. Mol. Cell. Biol. 2016, 36, 2794-2810


Oct 21 (Matsui) Moureau et al., A role for the p53 tumor suppressor in regulating the balance between homologous recombination and non-homologous end joining. Open Biol. 2016, 6, 160225


Oct 7 (Hashimoto) Tsouroula et al., Temporal and spatial uncoupling of DNA double strand break repair pathways within mammalian heterochromatin. Mol Cell 2016, 63, 293-305


Sep 16 (Iwabuchi) Cuella-Martin et al., 53BP1 integrates DNA repiar and p53-dependent cell fate decisions via distinct mechanis. Mol Cell 2016, 64, 1-14


Jul 22 (Sunatani) Renner et al., Annexin A2 enhances complement activation by inhibiting factor H. J Immunol. 2016, 196, 1355-1365


Jul 8 (Sakasai) Yeo CQ et al., p53 maintains genomic stability by preventing interference between transcription and replication. Cell Reports 2016, 15, 132-146


Jun 24 (Matsui) Pederiva et al., Splicing controls the ubiquitin response during DNA double-strand break repair. Cell Death Differ. 2016, doi:10.1038/cdd.2016.58


Jun 3 (Hashimoto) Jacquet et al., The TIP60 complex regulates bivalent chromatin recognition by 53BP1 through direct H4K20me binding and H2AK15 acetylation. Mol Cell 2016, 62, 409-421


May 20 (Iwabuchi) Tkac et al., HELB is a feedback inhibitor of DNA end resection. Mol Cell 2016, 61, 405-418


Apr 28 (Sunatani) Zhang et al., FBXW7 facilitates nonhomologous end-joining via K63-linked polyubiquitination of XRCC4. Mol Cell 2016, 61, 419-433


Apr 8 (Sakasai) Gelot et al., The cohesin complex prevents the end joining of distant DNA double-strand ends. Mol Cell 2016 , 61, 1-12


Mar 19 (Matsui) Zhang et al. A cell cycle-dependent BRCA1-UHRF1 cascade regulates DNA double-strand break repair pathway choice. Nat. commun. 2015, 7:10201, DOI: 10. 1038/ncomms10201 


Feb 26 (Hashimoto) Broderick et al. EXD2 promotes homologous recombination by facilitating DNA end resection. Nat. Cell Biol. 2016, 18, 271-280


Feb 12 (Iwabuchi) Liang et al. UHRF1 is a sensor for DNA interstrand crosslinks and recruits FANCD2 to initiate the Fanconi anemia pathway. Cell Rep. 2015, 10, 1947-1956


Jan 13 (Sakasai) Schwab et al. The Fanconi anemia pathway maintains genome stability by coordinating replication and transcription. Mol Cell 2016, 60, 1-11

Garcia-Rubio et al. The Fanconi anemia pathway protects genome integrity from R-loops. PLOS Genetics 2015, DOI: 10.1371/journal.pgen.1005674


Dec 11 (Matsui) ATM localization and heterochromatin repair depend on direct interaction of 53BP1-BRCT2 domain with gH2AX. Cell Rep. 2015, 13, 1-9


Nov 20 (Iwabuchi) Thorslund et al. Histone H1 couples initiation and amplification of ubiquitin signalling after DNA damage. Nature 2015, 527, 389-93


Nov 6 (Sunatani) Barazzuol et al. Low levels of endogenous or X-ray-induced DNA double-strand breaks activate apoptosis in adult neural stem cells. J Cell Sci. 2015, 128, 3597-3606


Oct 23 (Sakasai) Liu et al. LRF maintains genome integrity by regulating the non-homologous end joining pathway of DNA repair. Nat commun. 6, 8325


Oct 9 (Matsui) Alagoz et al. SETDB1, HP1 and SUV39 promote repositioning of 53BP1 to extend resection during homologous recombination in G2 cells. Nucleic Acids Res. 2015, 43, 7931-44


Sep 18 (Iwabichi): Xiong et al. 53BP1 promotes microhomology-mediated end-joining in G1-phase cells. Nucleic Acids Res. 2015, 43, 1659-70


Jul 17 (Sakasai): Sollier et al. Transcription-coupled nucleotide excision repair factors promote R-loop-induced genome instability. Mol Cell 2014, 56, 1-9


Jul 4 (Matsui): Peng et al. Modulation of LSD1 phosphorylation by CK2/WIP1 regulates RNF168-dependent 53BP1 recruitment in response to DNA damage. Nucleic Acids Res. 2015 May 20. pii: gkv528. [Epub ahead of print]


Jun 13 (Hashimoto): Jiang et al. Differential phosphorylation of DNA-PKcs regulates the interplay between end processing and end ligation during nonhomologous end-joining. Mol Cell 2015, 58, 172-85 


Jun 6 (Iwabuchi): Badie et al. BRCA1 and CtIP promote alternative non-homologous end-joining at uncapped telomeres. EMBO J. 2015, 34, 410-24


May 15 (Sunatani): Xiong et al. Stemness factor Sall4 is required for DNA damage response in embryonic stem cells. J Cell Biol. 2015  Mar 2;208(5):513-20.


Apr 25 (Sakasai): Xu et al. REV7 counteracts DNA double -strand break resection and affects PARP inhibition. Nature 2015 Mar 23


Apr 4 (Matsui): Gibbs-Seymour et al. Lamin A/C-dependent interaction with 53BP1 promotes cellular responses to DNA damage. Aging Cell. 2015 Apr;14(2):162-9.


Mar 28 (Hashimoto): Ceccaldi et al. Homologous-recombination-deficient tumours are dependent on Polθ-mediated repair. Nature. 2015 Feb 12;518(7538):258-62.


Mar 13 (Iwabuchi): Gatti et al. RNF168 promotes noncanonical K27 ubiquitination to signal DNA damage. Cell Rep. 2015 Jan 13;10(2):226-38.


Mar 7 (Sunatani): Poruchynsky et al. Microtubule-targeting agents augment the toxicity of DNA-damaging agents by disrupting intracellular trafficking of DNA repair proteins. Proc Natl Acad Sci U S A. 2015 Feb 3;112(5):1571-6.


Feb 27 (Sakasai): Xing et al. Interactome analysis identifies a new prologue of XRCC4 in non-homologous end joining DNA repair pathway. Nat Commun. 2015 Feb 11;6:6233.


Feb 14 (Matsui): Perfetti MT et al. Identification of a Fragment-like Small Molecule Ligand for the Methyl-lysine Binding Protein, 53BP1. ACS Chem Biol. 2015 Apr 17;10(4):1072-81.

Feb 14 (Matsui): Wang J et al. PTIP associates with Artemis to dictate DNA repair pathway choice. Genes Dev. 2014 Dec 15;28(24):2693-8.


Feb 07 (Hashimoto): Ouyang J et al. Noncovalent Interactions with SUMO and Ubiquitin Orchestrate Distinct Functions of the SLX4 Complex in Genome Maintenance. Mol Cell. 2015 Jan 8;57(1):108-22.


Dec 13 (Iwabuchi): Han X, Zhang L, Chung J, Mayca Pozo F, Tran A, Seachrist DD et al. UbcH7 regulates 53BP1 stability and DSB repair. Proc Natl Acad Sci U S A. 2014 Dec 9;111(49):17456-61.

Nov 08 (Sunatani): Dulev S, Tkach J, Lin S, Batada NN. SET8 methyltransferase activity during the DNA double-strand break response is required for recruitment of 53BP1. EMBO Rep. 2014 Nov;15(11):1163-74.

Nov 01 (Sakasai): Britton S, Dernoncourt E, Delteil C, Froment C, Schiltz O, Salles B et al. DNA damage triggers SAF-A and RNA biogenesis factors exclusion from chromatin coupled to R-loops removal. Nucleic Acids Res. 2014 Aug;42(14):9047-62.

Oct 25 (Matsui): Carr SM, Munro S, Zalmas LP, Fedorov O, Johansson C, Krojer T et al. Lysine methylation-dependent binding of 53BP1 to the pRb tumor suppressor. Proc Natl Acad Sci U S A. 2014 Aug 5;111(31):11341-6.

Sep 20 (Hashimoto): Larsen DH, Hari F, Clapperton JA, Gwerder M, Gutsche K, Altmeyer M et al. The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage. Nat Cell Biol. 2014 Aug;16(8):792-803.

Sep 13 (Iwabuchi): Ravishankar B, Shinde R, Liu H, Chaudhary K, Bradley J, Lemos HP et al. Marginal zone CD169+ macrophages coordinate apoptotic cell-driven cellular recruitment and tolerance. Proc Natl Acad Sci U S A. 2014 Mar 18;111(11):4215-20.

Sep 06 (Sunatani): Tavora B, Reynolds LE, Batista S, Demircioglu F, Fernandez I, Lechertier T et al. Endothelial-cell FAK targeting sensitizes tumours to DNA-damaging therapy. Nature. 2014 Oct 2;514(7520):112-6.

Jul 19 (Sakasai): Bhatia V, Barroso SI, Garcia-Rubio ML, Tumini E, Herrera-Moyano E, Aguilera A. BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2. Nature. 2014 Jul 17;511(7509):362-5.

Jul 12 (Matsui): Tuzon CT, Spektor T, Kong X, Congdon LM, Wu S, Schotta G et al. Concerted activities of distinct H4K20 methyltransferases at DNA double-strand breaks regulate 53BP1 nucleation and NHEJ-directed repair. Cell Rep. 2014 Jul 24;8(2):430-8.

Jun 28 (Hashimoto): Gupta A, Hunt CR, Hegde ML, Chakraborty S, Udayakumar D, Horikoshi N et al. MOF phosphorylation by ATM regulates 53BP1-mediated double-strand break repair pathway choice. Cell Rep. 2014 Jul 10;8(1):177-89.

Jun 09 (Sunatani): Katyal S, Lee Y, Nitiss KC, Downing SM, Li Y, Shimada M et al. Aberrant topoisomerase-1 DNA lesions are pathogenic in neurodegenerative genome instability syndromes. Nat Neurosci. 2014 Jun;17(6):813-21.

Mar 31 (Sakasai): Herrera-Moyano E, Mergui X, Garcia-Rubio ML, Barroso S, Aguilera A. The yeast and human FACT chromatin-reorganizing complexes solve R-loop-mediated transcription-replication conflicts. Genes Dev. 2014 Apr 1;28(7):735-48.

Mar 23 (Matsui): Shaltiel IA, Aprelia M, Saurin AT, Chowdhury D, Kops GJ, Voest EE et al. Distinct phosphatases antagonize the p53 response in different phases of the cell cycle. Proc Natl Acad Sci U S A. 2014 May 20;111(20):7313-8.

Mar 10 (Hashimoto): Orthwein A, Fradet-Turcotte A, Noordermeer SM, Canny MD, Brun CM, Strecker J et al. Mitosis inhibits DNA double-strand break repair to guard against telomere fusions. Science. 2014 Apr 11;344(6180):189-93.

Apr 19 (Sunatani): Lee DH, Acharya SS, Kwon M, Drane P, Guan Y, Adelmant G et al. Dephosphorylation enables the recruitment of 53BP1 to double-strand DNA breaks. Mol Cell. 2014 May 8;54(3):512-25.

Apr 12 (Sakasai): Aymard F, Bugler B, Schmidt CK, Guillou E, Caron P, Briois S et al. Transcriptionally active chromatin recruits homologous recombination at DNA double-strand breaks. Nat Struct Mol Biol. 2014 Apr;21(4):366-74.

Apr 05 (Matsui): Knobel PA, Belotserkovskaya R, Galanty Y, Schmidt CK, Jackson SP, Stracker TH. USP28 is recruited to sites of DNA damage by the tandem BRCT domains of 53BP1 but plays a minor role in double-strand break metabolism. Mol Cell Biol. 2014 Jun;34(11):2062-74.